This work focuses on prehistoric stratigraphic sequences as archaeological source. The first part summarizes and generalizes the history and results of the investigation of prehistoric and early medieval stratigraphic sequences from Bohemia and beyond. The second part considers the relation between settlement layer and the micro-relief, using the example of Prague 9-Miškovice. The third part is devoted to the analysis of the detailed excavation in 2001 of Late and Final Bronze Age settlement layers in Prague 10-Záběhlice. The author emphasizes the sometimes paradigmatically based dismissive relation to this type of archaeological source, the problems of excavations and risks at their interpretation.